Introduction
Ear bar surgery is a delicate procedure that is commonly performed on laboratory mice for various research purposes. This article aims to provide a detailed guide on how to mount mice in ear bar surgery, covering everything from preparation to post-operative care. It is important to note that this procedure should only be conducted by trained professionals in a controlled laboratory environment.
1. Equipment and Supplies
To perform ear bar surgery, ensure you have the following equipment and supplies ready:- Ether anesthesia system
- Microscope
- Ear bars (size appropriate for mice)
- Scalpel
- Forceps
- Suture materials
2. Preparing the Surgical Environment
Creating a sterile and controlled environment is crucial for the success of ear bar surgery. Follow these steps:- Disinfect and clean the surgical table
- Wear sterile gloves and gown
- Prepare a sterile field using sterile drapes
- Ensure proper lighting and microscope settings
3. Anesthesia
Anesthesia is necessary to ensure the mice do not experience pain or distress during the surgery. Here's how to administer anesthesia:- Place the mouse in an induction chamber with a controlled flow of ether
- Observe the mouse until it is fully anesthetized
- Transfer the anesthetized mouse to the surgical area
4. Mounting the Ear Bars
Properly mounting the ear bars is essential for stabilizing the mouse during the surgery. Perform the following steps:- Gently position the mouse in a prone position
- Apply a small amount of topical ointment to the ears to prevent drying
- Place the ear bars on each side of the head, ensuring a secure fit
- Tighten the ear bars gently to hold the mouse's head in place
5. Incision and Surgical Procedure
Once the mouse is properly positioned and secured, follow these steps for the incision and surgical procedure:- Clean the surgical area with an antiseptic solution
- Make a small incision using a sterilized scalpel
- Perform the required surgical procedure with precision
- Maintain appropriate hemostasis throughout the procedure
6. Wound Closure
After completing the surgical procedure, wound closure is necessary to ensure proper healing. Follow these steps:- Use sutures to close the incision, ensuring minimal tissue damage
- Regularly clean the wound with an antiseptic solution
- Apply a small amount of topical antibiotic ointment to prevent infection
- Monitor the wound for inflammation or signs of infection
7. Post-Operative Care
Proper post-operative care is crucial for the well-being and recovery of the mice. Consider the following:- Keep the mice in a controlled environment with access to food and water
- Monitor mice for any signs of pain, distress, or complications
- Administer analgesics as prescribed by the veterinarian
- Regularly clean and examine the surgical site
8. Average Price and Price Range
The cost of ear bar surgery for mounting mice can vary depending on various factors, including the specific research institution and the complexity of the procedure. On average, ear bar surgery can cost between $200 to $500 per mouse, including anesthesia, surgical materials, and post-operative care. However, it is important to contact the research facility or laboratory for an accurate estimate.FAQs
1. Is ear bar surgery painful for the mice?
No, ear bar surgery is performed under anesthesia, ensuring that mice do not experience pain or distress during the procedure.2. How long does the recovery process take?
The recovery process varies depending on the complexity of the surgical procedure. Mice typically require a few days to a week to fully recover.3. Are there any risks or complications associated with ear bar surgery?
While ear bar surgery is generally considered safe, there is always a risk of infection or complications. It is essential to follow proper aseptic techniques and monitor the mice closely after the procedure.References
- Kalueff AV, et al. Behavioral phenotyping of mice in pharmacological and toxicological research. Exp Opin Drug Metab Toxicol. 2007;3(6):873–886.
